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Regeneration through hrrea embryogenesis of an elite colombian Theobroma cacao L. Irrea embriones pasaron por fases que se correspondieron con medios de cultivo diferenciales: Las plantas enraizadas se llevaron a una mezcla de tierra: The embryos went through four phases; Induction, Formation, Maduration and Mantenimiento which corresponded each one with different media culture.

The developed plants were moved to a mix of potting soil and sand 1: The histological analysis showed the typical characteristics of the embryogenic development. Por otro lado, Guiltinan et al. Actualmente la cadena productiva colombiana de cacao enfrenta varios problemas, principalmente en el sector primario. Los protocolos fueron el desarrollado por Guiltinan et al.

Las fases de estos protocolos fueron: Se realizaron 3 repeticiones por tipo de explante para un total de explantes. Las condiciones de cultivo se mantuvieron constantes. De acuerdo con los trabajos realizados por Pruski et al. Un total de 67 plantas fueron usadas para este experimento.

Estos fueron procesados siguiendo el protocolo de Maximova et al. Sin embargo solo los embriones del clon BIOB alcanzaron la etapa cotiledonar. Las plantas de cm de altura se xe a condiciones ex vitro sin necesidad de un subcultivo previo en el medio de mantenimiento como lo describe el protocolo original de Fontanel et al.

En el pulso de 0. Por otro lado, Li et al.

Revista Colombiana de Biotecnología

Respecto a este evento, Quiroz et al. Por su parte Omokolo et al.

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Por otro lado, Von Arnold et al. Esto es coherente con los resultados obtenidos por Traore et al. En este ensayo se evaluaron como explantes dos estados de desarrollo de los embriones primarios, embriones tipo globular y embriones herramienfas cotiledonar. A parte de ello se encuentra la variabilidad propia de cada clon discutida anteriormente.

Factors affecting secondary somatic embryogenesis and embryo maturation in Morus alba L. Scientia Horticulturae A comparison between Theobroma cacao L. Zygotic embryogenesis and somatic embryogenesis from floral explants. Somatic embryogenesis and plant regeneration of tropical maize genotypes. Plant Cell Tiss Org Cult. Manual del cultivo de cacao. Ministerio de Agricultura, Programa para el Desarrollo de la Amazonia.

Effect of activated charcoal on Brassica oleracea microspore culture embryogenesis. Primary and secondary somatic herramietnas in leaf sections and cell suspensions of Coffea arabica cv.

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INCI30 Overview on perennial cultures. Plantation Research and Development, pp: Tissue culture studies on cocoa Theobroma cacao L.

A supplementation of current research. Direct sowing of Coffea arabica somatic embryos-produced in herramientqs bioreactor and regeneration of plants. Cryopreservation of cocoa Theobroma cacao L. Plant Science Use of secondary somatic embryos promotes genetic fidelity ds cryopreservation of cocoa Theobroma cacao L. Agricultural and Food Science Plan cacaoetero permitira al pais exportar cacao.

Regeneration of somatic embryos and roots from Quinche leaves cultured on media with different macroelements composition. Plant Cell, Herramientae and Organ Culture Development of Cassava Manihot esculenta Crantz.

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Agricultural uses of somatic embryos. Acta Horticulturae Somatic embryogenesis and plant regeneration from floral explants of cacao Theobroma cacao L. Plant Cell Tissue Organ Cult Tissue culture studies urrrea Theobroma cacao. A mineral nutrient formulation for microculture of woody plant species. Advances in cocoa Theobroma catwlogo propagation by somatic embryogenesis. Efficiency, genotypic variability, and cellular origin of primary and secondary somatic embryogenesis of Theobroma cacao L.

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Field performance of Theobroma cacao L. Revista Respuestas1: Somatic embryogenesis in Eucalyptus. A revised medium for rapid growth and bioassays with tobacco tissue cultures.

Physiol Plant, 15 3: La industria de Chocolates en Colombia. Characterisation of the cacao somatic embryogenesis receptor-like kinase SERK gene expressed during somatic embryogenesis.

Somatic Embryogenesis in Cacao Theobroma cacao. Biotechnology in Agriculture and Forestry Factors affecting maturation of avocado somatic embryos.

Factors affecting maintenance, proliferation, and germination of secondary somatic embryos of Eucalyptus globulus Labill. Plant Cell Tissue and Organ Culture Hystological changes and reseve acumulation during somatic embryogenesis in Eucalyptus globulus.

The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, Picomolar concentrations of salicylates induce cellular growth and enhance somatic embryiogenesis in Coffea arabica ureea culture. Plant Cell Rep Indirect somatic embryogenesis and morphohistological analysis in Capsicum chinense. Biologia65 3: Respuesta de Clones herramisntas Cacao Theobroma cacao L. Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree Cinnamomum camphora L.

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The role of activated charcoal in plant tissue culture. Biotechnology Herramkentas Micropropagation the Theobroma cacao using somatic embryo-derived plants.

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