Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.

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Our results indicate that AFLP seemed to be the best-suited molecular assay for fingerprinting and assessing genetic relationships among tropical maize inbred lines with high accuracy.

The RFLP assay reflects restriction size variation spread across the genome, because the use of RFLP markers resulted in the greatest average number of alleles per locus as compared to the other marker systems tested. Plant Mol Biol As expected, the PIC distributions revealed that, in terms of genetic distance, dominant markers had lower levels of polymorphism as compared to codominant markers.

Garcia I ; Luciana L. Jaccard similarities were calculated using version 2. Nucl Acids Res European Patent Application number: Correlations between genetic distances measured with different markers. Materials and methods Plant material and DNA isolation Eighteen S 3 selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR tenkii had their genetic distances surveyed using four different marker systems Lanza et al. In order to compare their relative efficiencies as markers and to find the most suitable marker for maize diversity studies we evaluated 18 inbred tropical maize lines using a number of different tekini as markers.

As expected, the magnitude of the coefficient of variation CV values decreased as the number of polymorphic loci bands evaluated increased.

Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)

To determine the sampling variance of the genetic distances produced by the different molecular data sets we performed bootstrap analysis using a decreasing number of loci for codominant markers or bands for dominant markers. Because the box-plots for each of our groups of samples were skewed we used the mean, median and maximum CV tekhii to determine the adequate number of polymorphic loci needed for acceptable precision.


Rev Bras Genet 4: Bootstrap analysis was used to verify if the number of polymorphic loci evaluated was high enough to provide accurate genetic distance estimates King et al. All of the 18 maize inbred lines studied by us had previously been investigated using the four different marker systems RAPD: The objectives of the study described in this paper was: We used the median and maximum coefficient of variation values to evaluate the accuracy of the genetic distance estimates because although the mean coefficient of variation is often used in the literature caution is needed when dealing with molecular marker data for which there is no assurance that the CVs values are distributed symmetrically.

Although similar average genetic distance values were obtained for the BR and BR intrapopulation crosses, the BR crosses showed the widest range of genetic distances with all of the four different markers assayed; probably because of the broader genetic base of the BR population.

Our results points to the need to adopt different strategies for selecting markers and choosing an upper number of SSR and RAPD markers. Tkenii the discrimination power of RFLPs in diversity studies has been well documented Smith et al. The application of SSR techniques to plants depends on the availability of suitable microsatellite markers, which have been developed for species such as soybean Rongwen et al.

SAS language guide for personal computers. For codominant markers, however, the distribution of values within each sample is skewed towards the higher values and it appears that mean or median CV values should be appropriate.

The results obtained in our study showed high agreement between Aflo and AFLP genetic distance estimates, such estimates having also been highly correlated in other studies Russell et al. J Genet Breed Although the AFLP markers gave the lowest mean PIC value they provided a similar degree of polymorphism information content to that provided by tenkii RAPD markers, which agrees with the results published by Becker et al.

The loci used were: Although this lower sensibility in detecting informative genotypic classes might be associated with the inability to distinguish heterozygotes from homozygotes because aglp binary scored AFLPs, Gerber et al.

Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines

Calculations were made using the following formula: However, we also found that SSRs markers gave a more heterogeneous distribution for individual PIC values than RFLP markers, although this might have been due to the low number of polymorphic loci evaluated for this marker Barbosa et al.


Indeed, we afl; that the RFLP and AFLP markers avlp sufficient numbers of polymorphic bands to produce reliable genetic distance estimates with high correlations between these two marker systems, the similarity between the results being explainable by the fact that they are similar techniques based on restriction site changes.

The RAPD markers were clearly the most distinct type of marker because the correlation values involving this marker were equal to or lower than 0. The results obtained based on the adjusted functions except for the mean CV shown in Figure 3 are presented teknki Table 3.

I ; Anete P. Briefly, a total of clone-enzyme combinations were analyzed, the maize genome being saturated 20 cM intervals with afkp least one RFLP probe selected by its map location on tenii chromosome. Even though the CV values were not low enough to indicate a high level of precision the SSR markers produced high, and the RAPD markers moderate, correlations between the genetic distance estimates and hybrid performance and heterosis for the BR intrapopulational crosses.

Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.

We used the median CV value to calculate the following: The past limitations associated with pedigree data and morphological, physiological and cytological markers for assessing genetic diversity in cultivated and wild plant species have largely been circumvented by the development of DNA markers such as restriction fragment length polymorphisms RFLPs; Botstein et al.

However, in the case of maize, tropical and temperate populations differ from each other because tropical af,p usually originate from composites with higher genetic variability and, most of the time, it is difficult to allocate tropical composites to well-defined heterotic groups by phenotypic evaluation.